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Plant cells are surrounded by a carbohydrate-rich compartment called the cell wall. This compartment plays a central role in growth, development, sexual reproduction and defence (Vicre et al., 2005; Mollet et al., 2007; Hematy et al., 2009; Seifert & Blaukopf, 2010). It is a carbohydrate-based structure composed mostly of polysaccharides (~90%) and glycoproteins (~10%). Polysaccharides include cellulose, pectin domains such as homogalacturonans (HG) with different levels of methylesterification, xylogalacturonan, rhamnogalacturonan-I (RG-I) and rhamnogalacturonanII (RG-II) and hemicelluloses (depending on the species, xyloglucan [XyG] or arabinoxylan) (Liepman et al., 2010), whereas cell wall proteins are either enzymatic such as glucanases (Gilbert, 2010) or nonenzymatic such as arabinogalactan proteins (AGP) (Ellis et al., 2010, Driouich & Baskin, 2008).

Immuno-Glyco-Imaging in Plant Cells: Localization of Cell Wall Carbohydrate Epitopes and Their Biosynthesizing Enzymes