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Advances in biochemistry-based analysis of biotic systems depend on improved procedures for the sorting of the non-covalently joined macromolecular assemblies that are obtained by the expelling of cellular contents. The development of preparative ultracentrifugation, for example, made possible the discovery of both ribosomes and various intracellular organelles (reviewed in Alberts et al., 2002). Some biotic systems depend on assembly more than one might conclude at first (reviewed in Kurakin, 2007). Improving this biochemistry-based analysis is an ongoing process, given that (1) multi-molecular, assembly-derived mechanisms are not yet understood, including (especially) the mechanisms of biological motors (reviewed in Howard, 2009; Myong & Ha, 2010) and (2) one reasonably projects that components of current biotic systems have abiotic ancestors that were also non-covalently joined assemblies, the understanding of which is potentially essential for understanding the origins of life (Koonin, 2009; Serwer, 2011).

Gels for the Propagation of Bacteriophages and the Characterization of Bacteriophage Assembly Intermediates