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In the mid-1970s, a new disease entity termed ‘non-A, non-B’ (NANB) hepatitis was first described and, in the following years, led to discovery of the causative virus, posttransfusion, and to community-acquired NANB hepatitis increasingly becoming recognized as a potentially serious disease that results in liver cirrhosis and/or hepatocellular carcinoma.16, 26 Hepatitis C virus (HCV) was first identified in 1989 using molecular methods at the Chiron Corporation, but to date, the virus has never been visualized or grown in cell culture.37 Hepatitis C virus (HCV) is a single-stranded RNA virus with a genome of about 10 000 nucleotides containing a single large, continuous open reading frame and with organization most closely resembling the Flaviviridae.11 HCV is a global healthcare problem and the World Health Organization (WHO) estimates that at least 170 million people (3 % of the world’s population) are infected with HCV worldwide and most of the patients are concentrated in developing countries. 48

An Overview of the Laboratory Assay Systems and Reactives Used in the Diagnosis of Hepatitis C Virus (HCV) Infections