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Several and distinct physiological processes in all the life forms are dependent on proteases, as processing and turnover of endogenous proteins, digestion of food proteins, regulation of formation and lysis of the clots, activation of apoptosis pathways, plant germination, sporulation, hormone activation, translocation through membranes, fertilization, control of immune response, cell differentiation and growing (Bode & Huber, 2000; Chou & Cai, 2006; Turk et al., 2000). Proteases are also involved in replication and propagation of infectious diseases, and the imbalance of their activity can cause important pathological disorders as inflammation, stroke, cancer and parasite infection (Chou & Cai, 2006; Johansson et al., 2002; Powers et al., 2002).

Affinity Chromatography as a Key Tool to Purify Protein Protease Inhibitors from Plants