English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Although our understanding of DNA methylation mechanisms and functions has vastly improved over the past two decades, the entire spectrum of influences of this potent mechanism is not fully grasped yet. Today, a wide variety of techniques are used to examine DNA methylation patterns but most of these methods are still relatively expensive and many are platform specific (Prokhortchouk and Defossez, 2008). The need has arisen to develop an economically viable and uncomplicated technique for DNA methylation analysis. The comet assay (single cell gel electrophoresis or SCGE) is a cost-effective, sensitive and simple technique which is traditionally used for analysing and quantifying DNA damage in individual cells (Azqueta, et al., 2011, Fairbairn, et al., 1995). By modifying this assay to be methylation sensitive, global DNA methylation can be routinely measured in cell cultures while simultaneously being able to deduce the integrity of the genetic material of the examined cells. The methylation sensitive comet assay was used to examine the effect of the accumulating metabolite, succinylacetone (SA), in hereditary Tyrosinemia type I (HT1), since hepatocellular carcinoma (HCC) frequently develops into this disease.

Investigating the Role DNA Methylations Plays in Developing Hepatocellular Carcinoma Associated with Tyrosinemia Type 1 Using the Comet Assay