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Despite that the narcotics had generated a regrettable stigma for Colombia, our country does not have a deep knowledge about the narcotic chemistry. In this study it is show the analysis of cannabinoids in the Colombian illicit crops of Cannabis sativa L. using Gas Chromatography (GC), Figure 1. The main important cannabinoids from C. sativa were identified by Gas Chromatography-Mass Spectrometry (GC-MS). The conditions for extraction and quantification of these cannabinoids (cannabidiol (CBD), Δ9tetrahydrocannabinol (THC) and cannabinol (CBN)) from C. sativa leaves were optimized by the response surface analysis-RSA, using as variables the extraction time and the extraction numbers. Then, the better sample amount and the best detection method were determined, after the validation of the GC analytic method was made. Additionally, the cannabinoids of samples of different Colombian geographic regions were analyzed, determining that the THC content vs THC/CBN content can serve as a discriminating factor to distinguish the geographic origin of the samples studied. Finally, it was showed that the GC is a good analytic tool for forensic chemistry in Colombia.

Gas Chromatography in Forensic Chemistry: Cannabinoids Content in Marijuana Leaves (Cannabis sativa L.) from Colombia