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There is a trend to preserve the plant germplasm by not only conventional ex situ methods or in vitro techniques, but also, more recently, by cryopreservation. Cryopreservation techniques are based on the storage of plant samples at very low temperature at which practically no chemical reactions occur and consequently, neither aging nor genetic changes of plant material. There has been a great development progress of cryopreservation methods during last years. Cryopreservation becomes a highly utilized technique for germplasm conservation. Generally the cryopreservation is storage of the samples. The samples can be e.g. organs and shoots tips from in vitro culture, or from the field, such as mature, immature bulbils, cloves of garlic or dormant buds of fruit trees, at the ultra-low temperature (mainly –196 °C, the temperature of liquid nitrogen).

Comparison of Cryopreservation Methods of Vegetatively Propagated Crops Based on Thermal Analysis