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Mycobacterium bovis is a member of the M. tuberculosis complex (MTC), a group of species (Mycobacterium tuberculosis, M. bovis, M. africanum, M. microti, and M. canetti) with a high genetic homology. M. bovis is the causative agent of tuberculosis in a range of animal species and humans, with worldwide annual losses to agriculture of $3 billion. The human burden of tuberculosis caused by the bovine tubercle bacillus is still largely unknown. M. bovis was also the progenitor for the M. bovis bacillus Calmette–Guérin vaccine strain, the most widely used human vaccine. Garnier et al. (2003) described the 4,345,492-bp genome sequence of M. bovis AF2122/97 and compared it with the genomes of Mycobacterium tuberculosis and Mycobacterium leprae. Strikingly, the genome sequence of M. bovis is >99.95% identical to that of M. tuberculosis, but deletion of genetic information has reduced the genome size.

intech ebooks

Molecular Techniques for Identification of Species of the Mycobacterium tuberculosis Complex: The use of Multiplex PCR and an Adapted HPLC Method for Identification of Mycobacterium bovis and Diagnosis of Bovine Tuberculosis