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Despite the significant progress, the post-thaw viability and fertility of the cryopreserved sperm are still reduced, as a consequence of accumulated cellular injuries that arise throughout the cryopreservation process. Many laboratory tests have already been carried out to verify these detrimental effects and their origin. Their is needed to well understand the whole process of cryopreservation and its influence on sperm function. As a consequence, it would lead to a subsequent improvement of sperm viability by means of reformulated protocols and approaches helping to minimize the detrimental effect of cryopreservation.

Methods of Assessment of Cryopreserved Semen