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Fungi of the genus Fusarium are worldwide occurring plant pathogens which cause severe damages to numerous cultivable plants (Weiland et al., 2000; Mirete et al., 2004; Youssef et al., 2007; Li et al., 2008) with the highest economical losses upon infection of maize, wheat and barley (Windels et al., 2000; Nganje et al., 2004). Fusarium caused diseases can destroy crops within several weeks and the infection leads to quality losses in two different aspects: besides the reduced yield due to reduced kernel size, the fungus produces various toxic metabolites while colonizing the plant. These mycotoxins heavily impair the quality of the harvest (McMullen et al., 1997). The acute or chronic toxicity of Fusarium released compounds led to the introduction of national or international limits to regulate mycotoxin levels in food and feed (e.g. limits of the European Community since 2006).

Novel Methods for the Quantification of Pathogenic Fungi in Crop Plants: Quantitative PCR and ELISA Accurately Determine Fusarium Biomass