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Proteins were first described in 1838 by the Dutch chemist Gerardus Johannes Mulder and named by the Swedish chemist Jons Jacob Berzelius. 1 The term protein comes from the Greek word proteios, meaning "primary". 2 Protein carries primary functions of the organisms, including both structural and physiological functions. One of the appropriate samples of its importance is the enzyme, which catalyzes almost all biochemical process in vivo. 3 Actually, lots of the most vital biological functions of an organism are fulfilled by proteins, so the isolation and expression of proteins become an important issue for both research and application.

The Denaturing and Renaturing are Critical Steps in the Purification of Recombinant Protein in Prokaryotic System