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Research projects in life sciences aim at studying and better understanding biological systems. Over the past 50 years, tremendous advances in molecular biology and biochemistry have provided essential tools to dissect biological processes down to the molecular level. Most of the time, when studying the structure and function of proteins, obtaining sufficient quantities of the native form of the protein isolated from the relevant cells or tissue is not feasible. The development of recombinant DNA technologies to clone and express genes encoding proteins of interest has revolutionized the design and execution of research projects (Cohen et al., 1973). Indeed, access to purified recombinant proteins enables a wide spectrum of studies, ranging from structural characterization of proteinproteinand protein-nucleic acid interactions to immunization programs to generate antibodies as research tools. The availability of sufficient quantities of purified recombinant proteins is often key to success. Furthermore, recombinant approaches for the production of proteins have profoundly impacted biomedical research and drug development as they have opened the possibility of producing clinical grade proteins as drugs. This has subsequently paved the way for the emergence and fast development of protein biologics that today represent a very successful and quickly expanding class of drugs (Saladin et al., 2009; Chiverton et al., 2010).

Episomal Vectors for Rapid Expression and Purification of Proteins in Mammalian Cells