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Salmonella is an important foodborne pathogen causing significant public health concern, both domestically and internationally (Tirado and Schmidt, 2001; Scallan et al., 2011). According to the latest CDC report Salmonella infections affect millions of people every year accounting for 11%, 35% and 28%, of illnesses, hospitalizations and deaths, respectively of the total U.S. foodborne diseases caused by all known foodborne pathogens (Scallan et al., 2011). Among those non-typhoid salmonellosis, S. Enteritidis (SE) has emerged as a major egg-associated pathogen. SE transmission to humans has been linked mainly to consumption of contaminated foods containing undercooked eggs (Rabsch et al., 2000). Fresh shell-eggs can be contaminated easily with SE through cracks in the shell by contact with chicken feces or by transovarian infection (Snoeyenbos et al., 1969). Consequently, the increase of consumption of shell eggs and egg products per capita in the United States to approximately 249 eggs per year (American Egg Board, 2008) may have contributed, in part, to increases in foodborne outbreaks (Altekruse et al., 1997), including a large multistate SE outbreak of SE outbreak associated with eggs in the US in 2010.

Multiplex TaqMan Real-Time PCR (qPCR) Assay Targeting prot6E and invA Genes for Fast and Accurate Detection of Salmonella Enteritidis