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Until relatively recently, the only way non-invasively to study brain cell membrane phospholipids in humans in vivo has been through the quantification of membrane phospholipid metabolism by means of ascertaining the levels of phosphomonoesters and phosphodiesters from 31-phosphorus neurospectroscopy scans. However, this only gives an indirect measure of the cell membrane phospholipid molecules. Ideally, one would want to study the brain cell membrane motion-restricted phospholipids directly, but it has previously proved almost impossible to obtain high-resolution magnetic resonance spectra of membrane phospholipids in living tissues owing to the large chemical-shift anisotropy of the 31-phosphorus confined to what is, in terms of nuclear magnetic resonance, the relatively rigid structure of the cell membrane, whether this be the external cell membrane or the membrane of an intracellular organelle.

The Use of 31-Phosphorus Magnetic Resonance Spectroscopy to Study Brain Cell Membrane Motion- Restricted Phospholipids