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The mangrove ecosystems have the capacity to act as a sink of heavy metals entering aquatic ecosystems. Despite their potential exposure to metal contaminated sediments, mangroves appear to be highly tolerant to heavy metals. In this study, we cloned metal tolerance gene from mangrove plant. Using CTAB method, RNA were isolated from leaves and root tissue of Rhizophora stylosa habitated at Weno island in Micronesia Chuuk lagoon using CTAB method and phytochelatin synthase 1 (PCS1) gene was cloned using gene specific primers. Expression of PCS1 gene was increased 1.91 fold and 2.72 fold in mangrove propagules exposed to 100 ppb Cd and 10 ppb Cu, respectively. These results indicate that expression of PCS1 gene are promising tools for health assessment of mangrove ecosystem. Key Words : Biomarker, Cadmium, Copper, PCS1, Rhizophora stylosa   (PE99161)  . * Corresponding Author : Taek-Kyun Lee(KIOST) Tel: +82-55-639-8630 email: tklee@kiost.acReceived March 29, 2013 Revised May 6, 2013 Accepted June 7, 2013

Cloning and Expression of Phytochelatin Synthase 1 Gene from Rhizophora stylosa Exposed to Cadmium and Copper