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Staphylococcus aureus can cause a variety of infections, especially nosocomial. Its importance lies in the \udcombination of virulence, invasiveness and antibiotic resistance constituting therapeutic challenges. This \udstudy aimed to assess the genotype and phenotypic resistance profiles of samples of S. aureus isolated from \udhospitalized patients in a Brazilian teaching hospital. We evaluated 1078 samples obtained from surveillance \udcultures and clinical S. aureus in hospitalized patients. To assess the phenotypic resistance profile was used \uddisk diffusion method according to CLSI criteria, 2011. For the determination of genotypic resistance was the \udpresence of the mecA gene by polymerase chain reaction - PCR. Of the 1078 samples tested \udphenotypically, it was observed that 75.1% of the samples were Methicillin Resistant Staphylococcus aureus \ud(MRSA), and of these, 98.4% were resistant to oxacillin and 100%, cefoxitin. To determine genotype was \udperformed for the PCR amplification of the mecA gene. Of the 443 samples tested for the mecA 336 samples \udwere positive (75.8%). Of these samples, 85.7% showed resistance phenotype oxacillin and cefoxitin 88.4%. \udDue to the high rate of MRSA, concludes the need for investment in research, rational use of antimicrobials \udand creation of reference laboratories for verification of antimicrobial resistance. \ud\u

colloquium vitae

AVALIAÇÃO FENOTÍPICA E GENOTÍPICA DO PERFIL DE RESISTÊNCIA DE AMOSTRAS DE Staphylococcus aureus ISOLADAS DE CULTURAS CLÍNICAS E DE VIGILÂNCIA DE UM HOSPITAL DE ENSINO BRASILEIRO