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A method for the calculation of whey protein fraction was developed for milk-based infant formula products, based upon an amino acid ratio calculated from amino acid profile data. Total amino acid data from 21 commercially available (five casein and 16 whey) commodities were used as the reference data from which the calculation model used in the validation study was built. This method has been evaluated for precision (repeatability, intermediate precision) and accuracy on nonfat dry milk and products manufactured with targeted whey levels of 32–63%, including National Institute of Standards and Technology infant formula Standard Reference Material (1849a). Estimates of repeatability were between 0.3 and 2.5% while those for intermediate precision were between 2.6 and 3.4%. Average recovery ranged from 97 to 100%. Additional reference material data are being gathered for better estimation of casein and whey amino acid reference values.

Calculation of Whey Protein Fraction in Milk-Based Infant Formula: First Action 2012.07