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Cationic amino acid transporter (HGMW-approved gene symbol SLC7A9, solute carrier family 7, member 9) plays a crucial role in amino acid nutrition. In the present study, we describe the cloning and sequencing of porcine . Based on the sequence of porcine deposited in the NCBI (National Center for Biotechnological Information), we identified a putative porcine homologue. Using rapid amplification of cDNA ends (RACE), the full-length cDNA encoding porcine was isolated. The porcine cDNA was 1,680 bp long, encoding a 487 amino acid trans-membrane protein. The predicted amino acid sequence was found to have 88.9% and 87.1% identity with human and mouse , respectively. Real-time RT-PCR indicated porcine transcripts expressed in heart, kidney, muscle and small intestine. The small intestine had the highest mRNA abundance while the muscle had the lowest (p mRNA abundance while the colon had the lowest (p mRNA level was highest on day 7 and 90 in the duodenum (p0.05) and had the highest abundance on day 60 (p0.05). The strongest expression appeared on day 7 in the ileum before weaning, and then decreased till day 30 but rose gradually again from day 60 to 150 (p

Molecular Cloning, Tissue Distribution and Segmental Ontogenetic Regulation of b0,+ Amino Acid Transporter in Lantang Pigs