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The percentages of sperm motility and normal acrosome on the liquid boar semen diluted and preserved at 4°C with lactose hydrate, egg yolk and N-acetyl-D-glucosamine (LEN) diluent were significant differences according to preservation day and incubation time, respectively. The sperm motility steadily declined from 96.9% at 0.5 h incubation to 78.8% at 6 h incubation at 1 day of preservation. However, the sperm motility rapidly declined after 4 day of preservation during incubation. The normal acrosome steadily declined from 93.3% at 0.5 h incubation to 73.8% at 6 h incubation at 1 day of preservation. However, the normal acrosome rapidly declined after 3 day of preservation during incubation. The rates of sperm penetration and polyspermy were higher in 5 and 10×10 6 sperm/ml than in 0.2 and 1×10 6 sperm/ml. Mean numbers of sperm in penetrated oocyte were highest in 10×10 6 sperm/ml compared with other sperm concentrations. The rates of blastocysts from the cleaved oocytes (2-4 cell stage) were highest in 1×10 6 sperm/ml compared with other sperm concentrations. In conclusion, we found out that liquid boar sperm stored at 4°C could be used for in vitro fertilization of pig oocytes matured in vitro. Also, we recommend 1×10 6 sperm/ml concentration for in vitro fertilization of pig oocytes. (Asian-Aust. J. Anim. Sci. 2004. Vol 17, No. 10 : 1369-1373)

Liquid Boar Sperm Quality during Storage and In vitro Fertilization and Culture of Pig Oocytes