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effect on subsequent maturation of GV stage pig oocytes after centrifugation. Therefore, a reliable RCF parameter to permit visualization of the GV without compromising the subsequent maturation or developmental competence of pig oocytes is necessary. In this study, we had optimized the condition for GV visualization in pig oocytes. The effects of different RCFs on subsequent maturation rates were also examined systematically. ABSTRACT : Pig oocytes contain high levels of lipids in the ooplasm, which reduces the visibility of the germinal vesicle (GV) under microscopic examination. Therefore, the purposes of this study were to investigate the effects of relative centrifugation force (RCF) on the visibility and maturation rates of the GV stage oocytes after centrifugation. In Experiment 1, cumulus-oocyte-complexes (COCs) were collected from slaughterhouse ovaries and randomly allocated to different RCFs (3,000 rpm: 970 g; 6,000 rpm: 3,900 g; or 10,000 rpm: 10,840 g) for 10 or 20 min. Percentages of visible GV were 76-79% in the oocytes centrifuged with 10,000 rpm, which were significantly higher (p<0.01) than those with 3,000 and 6,000 rpm. No significant differences in GV visibility were observed among oocytes with different lengths of centrifugation (p<0.05) regardless of the RCFs. In esperiment 2, the maturation rate of the oocyte was found significantly lower in the 20 min than in the 10 min group received 10,840 g of RCF (30 vs. 75%, p<0.05). In conclusion, the GV of porcine oocytes can be clearly visible by centrifugation at 10,840 gfor 10 min without compromising their subsequent maturation rates and a longer centrifugation time (20 min) had no beneficial influence on the visibility of GV stage pig oocytes. (Asian-Aust. J. Anim. Sci. 2004. Vol 17, No. 9 : 1227-1231)

The Relative Centrifugation Force Permits Visualization of the Germinal Vesicle in Pig Oocytes