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Production of alpha-1,3-galactosyltransferase ( α GT)-deficient pigs is essential to overcome xenograft rejection in pig-to-human xenotransplantation. However, the production of such pigs requires a great deal of cost, time, and labor. Heterozygous α GT knockout pigs should be bred at least for two generations to ultimately obtain homozygote progenies. The present study was conducted to produce α GT-deficient miniature pigs in much reduced time using mitotic recombination in neonatal ear skin fibroblasts.

Alpha-1,3-galactosyltransferase-deficient miniature pigs produced by serial cloning using neonatal skin fibroblasts with loss of heterozygosity