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PLASMA MEMBRANE-RICH FRACTIONS WERE PREPARED FROM MAIZE (ZEA MAYS L.) COLEOPTILES BY LOW SHEAR HOMOGENIZATION AND DIFFERENTIAL AND SUCROSE GRADIENT CENTRIFUGATION. PLASMA MEMBRANE FRAGMENTS WERE IDENTIFIED USING A SPECIFIC CYTOCHEMICAL STAIN. IN A COMPARISON OF 10 DIFFERENT CELL FRACTIONS OF VARYING PLASMA MEMBRANE CONTENT, THE N-L- NAPTHYLPHTHALAMIC ACID (MPA)-BINDING ACTIVITY OF THE FRACTIONS WAS DIRECTLY PROPORTIONAL TO THE CONTENT OF PLASMA MEMBRANE. THE NPA-BINDING APPEARS TO BE STRONG, BUT NON- COVALENT. NPA IS KNOWN TO INHIBIT AUXIN TRANSPORT EFFICIENTLY AND QUICKLY. THUS, THE RESULTS ARE CONSISTENT WITH THE LOCALIZATION OF AUXIN TRANSPORT SITES AT THE PLANT PLASMA MEMBRANE. /AUTHOR/

Herbicide-Binding Activity of the Plasma Membrane from Grass Coleoptiles : Progress Report