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This study was conducted to investigate the effects of fishmeal replacement with acid-concentrated soybean meal (ACSBM) on growth performance, blood biochemistry, and ingredient digestibility in juvenile olive flounder Para-lichthys olivaceus. Six experimental diets were formulated to replace fishmeal protein with ACSBM at 0%, 20%, 30%, 40%, 50%, and 60% (designated ACSBM0, ACSBM20, ACSBM30, ACSBM40, ACSBM50, and ACSBM60, respectively). Triplicate groups of fish (initial fish mean weight: 14.3±0.03 g) were fed the experimental diets to apparent satiation (twice daily at 08:00 and 18:00 h). After a 12-week feeding trial, a total of 180 healthy fish were randomly distributed into three Guelph system tanks at a density of 60 fish/tank (initial fish mean weight : 50.6±2.4 g) to test the apparent digestibility coefficients of the ingredients (ACSBM, fishmeal, and soybean meal). Although negative effects were observed with ACSBM40, ACSBM50 and ACSBM60 after 12 weeks of feeding, up to 20% of the fishmeal protein could be successfully replaced with ACSBM without significant growth depression. Hemoglobin and hematocrit values of fish fed the ACSBM50 and ACSBM60 diets were significantly lower than those of fish fed the ACSBM0 diet. Glucose values of fish fed the ACSBM60 diet were significantly higher than those of fish fed the ACSBM0 and ACSBM20 diets. Digestibility of protein in ACSBM and soybean meal was 85.9% and 82.5%, respec -tively. Results indicated that at least 20% of fishmeal protein can be replaced by ACSBM in diets of juvenile olive flounder without supplementation of limiting amino acids. Key words: Olive flounder, Fish meal, Acid-concentrated soybean meal, Digestibility, Replacement

hangug susan haghoi ji/han-guk susan gwahak hoeji

Evaluation of Acid-concentrated Soybean Meal as a Fishmeal Replacement and its Digestibility in Diets for Juvenile Olive Flounder Paralichthys olivaceus