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The oil in mackerel muscle was extracted using an environment friendly solvent, supercritical carbon dioxide (SC-CO 2 ) at a semi-batch flow extraction process and an n -hexane. The SC-CO 2 was maintained at a temperature of 45 o C under pressures ranging from 15 to 25 MPa. The flow rate of CO 2 (27 g/min) was constant during the entire 2 h extraction period. The fatty acid composi-tion of the oil was analyzed using gas chromatography (GC). Significant concentrations of eicosapentaenoic acid (EPA) acid and docosahexaenoic acid (DHA) acid were present in the SC-CO 2 extracted oil. The oil extracted using SC-CO 2 exhibited increased stability compared with n -haxane extracted oil. Particles of mackerel oil together with the biodegradable polymer, polyethylene glycol (PEG) were formed using a gas saturated solution process (PGSS) with SC-CO 2 in a thermostatted stirred vessel. Different temperatures (45-55 o C), pressures (15-25 MPa) and a nozzle size 400 µm were used for PGSS with a 1 h reaction time. The stabil -ity of mackerel oil in the particles did not changed significantly.

Fatty Acid Composition and Stability of Extracted Mackerel Muscle Oil and Oil-Polyethylene Glycol Particles Formed by Gas Saturated Solution Process