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To identify the species of Trichogramma occurring in the corn fields of Korea as egg parasitoids of Ostrinia furnacalis, we sequenced the full-length of ITS2 nuclear rDNA from 112 parasitoids collected during this study. As a reference to distinguish species,we also retrieved full-length ITS2 sequences of 60 Trichogramma species from the NCBI GenBank database. On the basis of the size an d3′terminal sequence pattern of the ITS2 sequences, the Trichogramma samples collected in this study were divided into three groups (K-1,-2, and -3). Evolutionary distances ( d) within and between groups based on ITS2 sequences were estimated to be ≤0.005 and ≥0.080,respectively. In the net average distance between groups or species, the d value between K-1 and T. ostriniae , K-2 and T. dendrolimi , andK-3 and T. confusum was the lowest, with values of 0.016, 0.001, and 0.002, respectively. In the phylogenetic tree, K-1 and K-2 were clustered with T. ostriniae and T. dendrolimi , respectively. However, K-3 was clustered with three different species, namely, T. confusum, T. chilonis, and T. bilingensis. NCBI BLAST results revealed that parasitoids belonging to K-1 and K-2 showed 99% identity with T. ostriniae and T. dendrolimi , respectively. Parasitoids in K-3 collected from Hongcheon showed 99-100% identity with T. confusum and T.chilonis, and one parasitoid in K-3 collected from Gochang had 98% identity with T. bilingensis , T. confusum , and T. chilonis . On the basisof these results, we infer that the species of Trichogramma collected in this study are closely related to T. ostriniae (K-1) and T. dendrolim i(K-2). However, it was not possible to distinguish species of K-3 using the ITS2 sequence alone.Key words: Ostrinia furnacalis, Zea mays, Trichogramma, ITS2, Molecular phylogenetic tree :    Trichogramma           ITS2 DNA    .      NCBI GenBank   Trichogramma  60 ITS2    .    ITS2 DNA  3′     3 (K-1, -2, -3) .       (Evolutionary distance, d) 0.005      d (≥0.080) .   GenBank      K-1 T. ostriniae , K-2 T. dendrolimi , K-3 T. confusum  d   0.016, 0.001, 0.002   .   K-1T. ostriniae, K-2 T. dendrolimi   K-3 T. confusum, T. chilonis, T. bilingensis  . NCBI BLAST  K-1 T. ostriniae K-2 T. dendrolimi 99% identity .  K-3    T. confusum, T. chilonis 99-100% identity ,    T. bilingensis, T. confusum, T. chilonis 98% identity  .        K-1 K-2  T. ostriniae T. dendrolimi,    K-3 ITS2    .: , , Trichogramma , ITS2, *Corresponding author: seoby@korea.krReceived February 14 2014; Revised June 9 2014Accepted July 1 2014

han-guk eung-yong gonchung hakoeji/han'gug eung'yong goncung haghoeji

Molecular Identification of Trichogramma (Hymenoptera: Trichogrammatidae) Egg Parasitoids of the Asian Corn Borer Ostrinia furnacalis, Based on ITS2 rDNA Sequence Analysis