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Mixed vesicles including phosphatidylcholine (PC), phosphatidyl serine (PS) and amphoteric crown ether compound (C18-crown-6) with different component fractions were prepared and their ion-complexing phenomena examined by measuring their electrophoretic mobilities in different salt solutions. The crown ether compounds retained their selectively ion-complexing ability for some special cations (K+ and Ba2+) even on the vesicle surfaces. The zeta-potentials of the mixed vesicles of PC+PS+crown were determined extensively by fraction of the PS component and variation in metal cations in aqueous solution. That is, this parameter shifted remarkably in a negative direction with increase in the PS-fraction only in salt solutions containing K+ or Ba2+. No shifting of zeta-potentials could be detected in the case of (i) PC+PS vesicles without crown ether or, (ii) salt solutions that did not complex with the crown ether (Li+ or Ca2+).

Interfacial Electrochemical Studies on Ion-complexing Phenomena of Mixed Phospholipid Vesicles Including Crown Ether Compounds