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This study was conducted to determine why 1, 2-; 2, 1-and 1, 2-; 1, 3-positional isomers of diacyl glycerolipids , including those that are depolarized, are separated on reverse phase HPLC while triglycerides are not . Interaction forces among the carbon chains of lipid molelcules generated by the Coulomb force of atoms were concluded to be stereochemically well balanced in the case of triglycerides in contrast to diacyl glycerolipids . Possibly, the larger the acyl carbon number, the greater is the lack of conformity of interaction forces among acyl moieties. This is a factor determining the chemical potential of lipid molecules and gives rise to small but reproducible differences in retention on reverse phase HPLC between the 1 , 2-and 2, 1-as well as 1, 2-and 1, 3-positional isomers.

Chromatographic Behavior of Glycerolipid with Respect to 1, 2-; 2, 1-and 1, 2-; 1, 3-Positional Isomers on Reverse Phase Mode