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When TBA method is applied to oxidized lipids, red color (about 530nm) and yellow color (about 450nm) are produced. The red color is rather stable and is used for the analysis, but the yellow color is unstable. An attempt was made to solve the mechanism of producing the yellow color by comparing the results of Sidwell's method (mainly red color) and Ottlenghi's method (2 colors). The reaction of hydroperoxides with TBA and the reaction under addition of Na2SO3 were also tested. From these experiments, the compound developing yellow color was deduced to be caused by an aldehyde which came from a primary decomposed product of hydroperoxides by further oxidative decomposition.

On the Reacting Compounds in the TBA Method for the Determination of Lipid Oxidation