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Bacillus subtilis RB14 produces the lipopeptide antibiotic iturin A by submerged and biofilm fermentation. In this study, we optimized the conditions for iturin A production in a jar fermentor. The maximum yield of iturin A was 932 mg L -1 after 120 h. The surface tension of water decreased from 72.0 to 39.0 mN m -1 as the concentrations of C 14 iturin A increased, indicating that C 14 iturin A behaves as a surfactant in water. The critical micellar concentration obtained from the intersection of two fitted lines was 1.2 × 10 -4 M. Moreover, the surface tension of water decreased as the length of the alkyl chain of iturin A increased.

Evaluation of Yield and Surface Tension-lowering Activity of Iturin A Produced by <i>Bacillus subtilis</i> RB14