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The aim of this study was to discriminate the authenticity of perilla oils distributed in Korea using their 1 H nuclear magnetic resonance (NMR) spectra acquired by a 43 MHz low-field benchtop NMR spectrometer. Significant differences existed in the integration values of all 6 peaks found in the spectrum between authentic and adulterated perilla oil samples. The integration values of 4 peaks that signify the methylene protons present in all fatty acids (FA) and allylic or olefinic protons present in all unsaturated FA were the best variables for establishing perilla oil authenticity. The procedure for applying the range of variables found in authentic perilla oil samples correctly discriminated between the samples of perilla oils with soybean oils added at concentrations of ≥ 6 vol%. The results demonstrated that this NMR procedure is a possible cost-effective alternative to the high-field 1 H NMR method for discriminating the authenticity of perilla oils.

A 43 MHz Low-Field Benchtop <sup>1</sup>H Nuclear Magnetic Resonance Method to Discriminate Perilla Oil Authenticity