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The factors which cause death of the living cells in a bifidobacterial preparation (Lac B) were studied. When the relative humidity exceeded 50%, or when the moisture absorption in the preparation exceeded 5%, the number of the living cells rapidly reduced at 27°. In such humidity, however, death of the cells was not observed during 10 days, when the temperature was maintainedat 4°. When the cells, dried over P2os, were compounded with the same quantity of a dried drug and stored under dry condition, the number of living cells did not reduced for 1 week even at 37°.

Factors Causing Death of Living Cells in Bifidobacterial Preparations