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A new method for the determination of bromvalerylurea (BVU) in the serum of acute toxipathic patients was developed.The serum was incubated with urease to hydrolyze urea which interferes with the determination of BVU. After deproteinization with trichloracetic acid, the sample solution was reacted with diacetylmonooxime, and the color developed was measured at 525nm.A straight calibration curve was obtained between 10 and 300μg BVU/ml serum.This method was simple and precise, and was applied to the acute toxipathic patient's serum.

A simple colorimetric determination of bromvalerylurea in acute toxipathic patient's serum.