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Received May 9, 2018 Accepted October 8, 2018 A simple spectrophotometric assay was developed for quantitative determination of selenium in selenium injection which is a hospital preparation for patients with selenium deficiency. Aliquots of the samples (500 μL) added 20 μL of 2.5 M formic acid and 20 μL of 18.5 mM O-phenylene diamine were incubated for two hours at room temperature. The absorbance was measured in the mixture at 333 nm by using NanoDrop, a convenient instrument for spectrophotometric assay. The standard curve for determining selenium concentration was linear in the range of 1.25-30 μg/mL (r > 0.999). Coefficients of variation for intraand inter-day assays were 0.3 to 5.5% and 0.8 to 2.8%, respectively. Assay validation was confirmed by five independent measurements of 50 μg/mL selenium injection in three pharmacists with the accuracy of 0.0 to 1.6%. This assay method was successfully applied to the quantitative determination of selenium in selenium injection.

A Simple Spectrophotometric Assay for Determination of Selenium Concentration in the Hospital Preparation