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Hybridomas were developed in 1975 as a stable platform for creating monoclonal antibodies (mAbs). Over the past 40 years, hybridomas have been a leading platform for creating mAbs, yet of the 50 commercial mAbs approved for sale by the US Food and Drug Administration (FDA), only 18 are produced using hybridomas. Of the remainder, 25 are produced using Chinese Hamster Ovaries (CHO), six with E. coli, and one using phage display. Since 2009, hybridomas have accounted for just one of 12 new mAbs approved by FDA. Recent advances in bioprocessing and bioinformatics show great promise in reinvigorating interest in hybridoma technology for commercial uses. In this paper, we review the history of hybridomas, hybridoma technology, and some downstream uses of hybridomas including the production of humanized and fully human mAbs. We also highlight some new technologies, such as HyLiTE (Hybrid Lineage Transcriptome Explorer) software to analyse hybridomas, gold nano-particle laser cell fusion, and distortion-based microfl uidics for selecting, fusing and growing hybridomas in one device. At present, there are no reviews available to researchers covering new developments in cell fusion or cell selection for hybridoma production. Technology available since 2014 can increase effi ciency rates of hybridoma production from ~1 in 10,000 cells to ~95%. The hybridoma platform has generated many bioproducts and bioprocesses and is ideal for commercial applications in the drug discovery, drug development and drug manufacturing paths.

Life begins at forty – hybridomas: ageing technology holds promise for future drug discoveries