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We experienced a case of congenital cytomegalovirus (CMV) infection exhibiting excretion of CMV at quite high titer (more than 50% Tissus Culture Infectious Dose 104.3) in urine and throat. Following this experience, the present study investigated the CMV excretion rate of children in the general pediatric ward of Chiba University Hospital, and investigated the process by which CMV becomes inactive in extracellular environment, in order to check the possibility of a nosocomial CMV infection. 10 children in the room where the case had been admitted and 11 children in the adjacent room were surveyed for excretion of the virus into their urine. 6 of the 21 children, 3 from each room, excreted CMV into their urine, and 3 of the 6 children excreted at more than 10 3 TCID50/ml Tests for the stability of CMV in urine, contrary to our expectations, showed that the stability of CMV was comparatively stable, i. e. the residual infectious CMV was about 10% of the starting material in urine after being kept for five days at room temperature. Similarly 90 % of CMV in urine exposed to the atmosphere was still active after 24 hours. These findings do suggest that CMV could be a possible candidate as agent of nosocomial infection in general pediatric wards, and so it seems that careful nursing practices, such as effective methods of urine disposal, sterilization of all materials contaminated with urine, and careful hand washing by all the personnel who come into contact with urine, to prevent nosocomial CMV infection are required. 1)千 葉 大 学 病 院 ,2)千 葉 大 学 看 護 学 部 受 理:昭 和61年7月23日,Accepted: July 23, 1986.

A Study Of the Cytomegalovirus Excretion Rate on the Children in a General Pediatric Ward and Its Inactivation Kinetics in Extracellular Environment