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In recent years enantioseparation of both active pharmaceutical ingredients and bio moleculessuch as amino acids became more and more necessary because in most cases the two stereo forms exhibitdifferent pharmacological effects. This article deals with the chiral separation of dansylated amino acidsby ligand-exchange capillary electrophoresis using L-phenylalaninamide, L-lysine and L-threonine as chiralselectors. Experiments with different central metal ions such as Cu(II), Co(II), Cd(II), Ni(II) and Zn(II)were carried out. Optimal conditions were found out by studying the effect of the pH and the selector molarityon the chiral resolution. Best separation was obtained for the Cu(II)/L-lysine complex, showing achiral resolution up to 17 for Dns-DL-Met. (doi: 10.5562/cca1762

croatica chemica acta

Enantioseparation of Dansylated Amino Acids by Ligand-exchange Capillary Electrophoresis Using L-phenylalaninamide, L-lysine or L-threonine as Chiral Selector