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A new simple, sensitive and LC-MS/MS method for quantification of spironolactone and its metabolite,canrenone, in human plasma is proposed. The analytes were analysed on a C18 column at 48 ºC,by using a mobile phase of 58 % methanol, 42 % 10 mmol dm−3 ammonim acetate in water and a flowrate of 1 mL/min. The detection of both analytes in plasma was performed as follows: ESI+, EIC (m/z169;187;283;305) from m/z 341, after protein precipitation with methanol. Calibration curves were generatedover the ranges 2.77–184.50 ng/mL for spironolactone and 2.69–179.20 ng/mL for canrenone by usinga weighted (1/y) linear regression. The absolute values of within- and between-run precision and accuracyfor both analytes ranged between 3.1 and 13.9 %, and the mean recovery was 99.7 %. The analytesdemonstrated good stability in various conditions. The validated method has been applied to a bioequivalencestudy of 50 mg spironolactone tablets on healthy volunteers. (doi: 10.5562/cca1761

Determination of Spironolactone and Canrenone in Human Plasma by High-performance Liquid Chromatography with Mass Spectrometry Detection