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Oregonin ((5S)-1,7-bis(3,4-dihydroxyphenyl)-5-(β-D-xylopyranosyloxy)-heptan-3-one) is the first discovered and reported naturally occurring diarylheptanoid glycoside. It exhibits high biological activity, but it is also the compound of interest because of its ability to form reddish-orange and reddish-brown colours. In this research, European black alder (Alnus glutinosa (L.) Gaertn.) bark was separately subjected to lyophilisation and oven-drying before oregonin extraction with two different solvents. According to the results, methanol has proven to be more suitable as solvent compared to deionised water for oregonin extraction by maceration from black alder bark matrix regardless of the dehydration method. Accordingly, in the case of methanol, much higher yields of oregonin were obtained after lyophilisation, than after oven-drying. Furthermore, extraction by deionised water produced slightly higher yield of oregonin after oven-drying than after lyophilisation, as opposed to methanol. However, in much lower oregonin concentrations. Among other things, oregonin propensity to degradation at elevated temperature could probably be applied for improving and facilitating alder wood hydrothermal processing by minimizing uneven discolouration.Oregonin ((5S)-1,7-bis(3,4-dihidroksifenil)-5-(β-D-ksilopiranosiloksi)-heptan-3-on) prvi je otkriveni i objavljeni prirodni diarilheptanoid glikozid. Pokazuje visoku biološku aktivnost, a važan je i zbog svoje sposobnosti da stvara crvenonarančaste i crvenosmeđe boje. U ovom je istraživanju kora europske crne johe (Alnus glutinosa (L.) Gaertn.) zasebno podvrgnuta liofilizaciji i sušenju u sušioniku prije ekstrakcije oregonina dvama različitim otapalima. Prema dobivenim rezultatima, pokazalo se da je za ekstrakciju oregonina maceracijom iz matrice kore crne johe prikladnije otapalo metanol nego deionizirana voda, bez obzira na primijenjenu dehidracijsku metodu. U skladu s navedenim, upotrebom metanola ostvareni su mnogo viši prinosi oregonina nakon liofilizacije nego nakon sušenja u sušioniku. Za razliku od metanola, pri ekstrakciji deioniziranom vodom prinosi oregonina nešto su veći nakon sušenja u sušioniku nego poslije liofilizacije, ali su njegove koncentracije znatno niže. Među ostalim, sklonost oregonina degradaciji pri povišenoj temperaturi vjerojatno bi se mogla iskoristiti za poboljšanje i olakšanje hidrotermičke obrade crne johe minimalizacijom neravnomjerne promjene boje

Influence of Lyophilisation and Oven-Drying on Extraction Yield of Oregonin from European Black Alder (Alnus glutinosa (L.) Gaertn.) Bark