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<p>This study was conducted to determine the effects of emulsifierand multi-enzyme in different energy density diet on growth performance, blood profiles, and relative organ weight in broiler chickens. A total of five hundred and forty2-d-oldmale Ross broilers (BW of 42.4 ±1.3 g) wereused in a 35-d experiment and randomly divided into 5 treatment groups: 1) NC [low energy diet, 3% tallow, ME = 3000 (1 to 21 d) and 3100 kcal/kg (22 to 35 d)], 2) PC [high energy diet, 5.5% soybean oil, ME = 3150 (1 to 21 d) and 3250 kcal/kg (22 to 35 d))], 3) P1 (NC+0.1% multi-enzyme), 4) P2 (NC + 0.05% emulsifier), 5) P3 (NC + 0.1% multi-enzyme + 0.05% emulsifier). Multi-enzymecontained ?-galactosidase, galactomannase, xylanase, and beta-glucanase. Emulsifier was a commercial product named Prosol<sup>® </sup>which wassodium stearoyl-2-lactylate.There were 9 replications per treatment with 12 birds per pen. From d 0 to 21, body weight gain (BWG) in PC and P1 treatments increased (P < 0.05) compared with NC treatment. From d 22 to 35, feed intake (FI) was greater (P < 0.05) in P3 treatment than PC treatment. On d 35, triglyceride concentration in PC, P1 and P3 treatments was greater (P < 0.05) compared with NC treatment. No differences were observed on white blood cell (WBC), red blood cell (RBC) and glucose concentration. The relative weight of the spleen was significantly decreased (P < 0.05) in P3 treatment compared with PC treatment. Furthermore, the relative weight of the bursa of Fabricius in P3 treatment was higher (P < 0.05) than PC, P1 and P2 treatments. In conclusion, the results of this study indicate that emulsifier andmulti-enzymein low-density diets can partially improve growth performance, triglyceride, and relative organ weight in broiler chickens, which can counterpart the negative effects caused by the decreased nutrient concentration.</p

journal of agricultural science

Effects of Emulsifier and Multi-enzyme in Different Energy Densitydiet on Growth Performance, Blood Profiles, and Relative Organ Weight in Broiler Chickens