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An imperative class of ?-amino acids bearing 1,2,4-triazinone and N ,C-disubstituted glycine ( 5a,b ) and/or the related systems ( 6 and 7 ) have been synthesized by the condensation of 6-(2'-aminophenyl-4-phenyl-3-thion -1,2,4-triazin-5-one ( 1 ) with the appropriate steroids, Epiandrosteron ( 2a ) and Dehydrosterone ( 2b ), followed by the addition of hydrocyanic acid. Nucleophilic substitution of mercapto group of ( 5 ) by 4-fluoroaniline and sulfanilamide give the corresponding systems 6 and 7 . Compounds 6,7, 5 , and 4 showed a high enzymatic effect as cellobiase agents against some tested fungi .

Synthesis of Novel a-Amino Acids Bearing 1,2,4-triazinone and Steroidal Moieties as Enzymetic Affect (Cellobiase Activity) Part I