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Phytochemical screening, antimicrobial properties and proximate analysis of the seeds of Landolphia Owariensis P. Beauv were investigated. Dried seeds of landolphia owariensis P. Beauv were pulverized and extracted with 96.5% ethanol using soxhlet extraction method. The phytochemical screening of the extract revealed the presence of glycosides, saponins and phenols while tannins, flavonoids, alkaloids and steroids were not detected. The crude ethanol extract showed antimicrobial potency against candida albicans , streptococcus faecali , escherichia coli , pseudomonas aeruginosa , staphylococcus aureus and coliform bacilli at the concentrations of 100 mg/mL. 50 mg/mL, 25 mg/mL and 16.5 mg/mL with inhibition zone diameter (IZD) values ranging between 16 mm-30 mm. The crude sample was partitioned into acidic, basic and neutral metabolites which showed varying degrees of antimicrobial potency at the concentrations of 100 mg/mL, 50 mg/mL, 25 mg/mL and 16.5 mg/mL against the same organisms. The acidic metabolite showed pronounced antimicrobial activity against these organisms compared to the basic and neutral metabolites. Amoxycillin (amoxyl) and orazone were used as standard antibiotics and levofloxacin as a standard fungicide. The proximate analysis of the untreated seed sample gave 2.27% ash content, 15.32% moisture content, 15.70% crude fiber, 28.06% crude protein and 48.45% carbohydrate.

Phytochemical Screening, Antimicrobial Properties and Proximate Analysis of Landolphia Owariensis P. Beauv Seeds