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To separate and purify anthocyanins from purple pitaya ( H. polyrhizus ) with SP825 resin, the adsorption properties of the SP825 resin were evaluated by static and dynamic experiments with respect to the following parameters: pH, temperature, dilution multiple of the sample solution and concentration of the ethanol eluting agent. The data from the static experiments were fitted to the pseudo-second-order kinetics model (R 2 = 0.9919) and Freundlich isotherm model (R 2 = 0.9672). A total of 658 mg of anthocyanins were contained in 1000 g of H. polyrhizus fresh fruits (both peel and pulp), which could be made into 1.5 g of anthocyanin-rich extract from H. polyrhizus (AEHP) containing 520 mg of anthocyanins. Overall, 79% of the anthocyanins were extracted. The AEHP showed strong DPPH radical scavenging activity, ABTS radical scavenging activity, hydroxyl radical scavenging activity, reducing power, ferrous metal ion chelating ability and SOD-like activity. Moreover, the AEHP inhibited the proliferation of the human colorectal cancer cell line DLD-1 and accelerated the growth of macrophage cells. Finally, the AEHP was proven to have a high tinctorial power and physiological and health functions.

international journal of biology/internationa journal of biology

Optimisation of Anthocyanin Extraction from Purple Pitaya and Verification of Antioxidant Properties, Antiproliferative Activity and Macrophage Proliferation Activity