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A cell line was established from embryo cells of rose bitterling Rhodeus ocellatus ocellatus (Teleostei: Acheilognathinae). This cell line is maintained for more than seven years in my laboratory. Karyotype in it does not have the change either. In maintenance of the biological diversity, it is an important problem to demand a method to prevent the extinction of the species. This cell line will become the clue of the study of the embryonic stem cell in endangered species. On the other hand, this cell line which karyotype is stable in its will be a useful tool for a mutagenicity assay.

A Cell Line Derived from Early Embryos of Rose Bitterling (Teleostei: Cypriniformes: Acheilognathinae)