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Free radicals, known as reactive oxygen species (ROS), are the products of normal cellular metabolism. A free radical can be defined as an atom or molecule containing one or more unpaired electrons. The odd number of electron(s) of a free radical makes it unstable, short lived and highly reactive.1-3 Free radicals are compounds that trigger the aging process and degenerative diseases. Antioxidants are compounds that prevent the formation of free radicals. Antioxidants are divided into two types, natural and synthetic antioxidant. Natural antioxidant commonly found in plants such as fruits, grains, and vegetables. Some natural antioxidant compounds are derivatives of flavonoids, phenol, ascorbic acid, coumarin, hydroxycinnamic, dihydroflavone, tocopherol, and catechin. Synthetic antioxidants include butyl hydroxylanisol, butyl hidroxytoluen, propyl gallate, ethoxyquin.4 Synthetic antioxidants such as butylated hydroxy aniline (BHA) and butylated hydroxy toluene (BHT) have been known to have large side effects including causing liver damage.5 This encourages the development of natural product to be a source of antioxidants. Natural product that can be used as antioxidants is green tea leaves (Camellia sinensis L). The antioxidant activity of green tea is caused by polyphenolic compounds, especially flavonoid groups.6,7 Catechin is flavonoids compound that contained in green tea leaves. The types of catechins that contained in green tea are epigallocatechin (EGC), epigallocatechin gallate (EGCG), epicatechin gallate (ECG), and gallate epicatechin (GEC).8-10

Antioxidant Activity of Ethanolic Extract and Various Fractions from Green Tea (Camellia sinensis L.) Leaves