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An ionic liquid (ILs) is a new alternative solvent (containing cations and anions) has the physical and specific solutions properties, and also had proven to be a promising substituent of conventional organic solvents are flammable, volatile and toxic in various processes. The ILS application in separating the secondary metabolites compound from Peperomia pellucida (L) Kunth herbaceous can be carried out using microwaveassisted extraction method (MAE). Objective: The aim of this study was to investigate the effect of ionic liquid based microwave-assisted extraction method for attracting the secondary metabolites compound from P. pellucida compared with a conventional solvent for the enrichment of secondary metabolites (especially polyphenols group) and their HPTLC fingerprinting profile. Methods: The herb dried powder extracted by microwave-assisted extraction using an ionic liquid such us 1-Butyl-3-methylimidazolium chloride ([BMIM]Cl), 1-Butyl-3-methylimidazolium Bromide ([BMIM]Br). The obtained extract solution was partitioned using an organic solvent; the extract was evaporated to obtain a dry extract and determination the HPTLC fingerprinting profile. Results: Based on the research results was obtained from the various types of solvents there are differences in the extraction of secondary metabolite.

Application of Ionic Liquid based Microwave-Assisted Extraction of the Secondary Metabolite from Peperomia pellucida (L) Kunth