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Aim: The aim of this research study was to develop a simple, rapid, precise, accurate and economical RP-HPLC method, with a simple mobile phase for the identification and determination of the antimicrobial peptide α-defensin-2. Materials and Methods: Separation was carried out at 25°C, using column Luna 5U (C18, 250x4.6, 5 μm) with mobile phase consisting of acetonitrile: water (40: 60 v/v). The detector was set at 210 nm. The flow rate was 1.2 ml/min and injection volume was 20 μl. Results: The developed method was validated as per ICH guidelines and the kinetic behavior of α-defensin 2 was studied in respect of different pH. Conclusion: The obtained data can serve for creating an in vivo monitoring program and quality control for different studies and preparations.

HPLC Method for Determination of the Chemical Stability of Antimicrobial Peptide α-Defensin 2