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With the aim of ascertaining the existence of a correlation between in vivo resistance to Verticillium albo-atrum and in vitro responses to the fungal cell wall components, peroxidase (PO) and phenylalanine ammonia lyase (PAL) were assayed using in vitro cultures of three alfalfa genotypes (resistant, intermediate resistant and susceptible). Calli derived from these three genotypes treated with V. albo-atrum cell wall components showed varying degrees of increase in PO and PAL activities. The differential responses of these enzymes to the fungal cell wall components coincided with genetic differences among the three genotypes.

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Alfalfa- Verticillium albo-atrum Interactions: II. In vitro Peroxidase and Phenylalanine Ammonia-Lyase Activities Enhanced by Treatment with Fungal Elicitors.