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*1: Two species of protoplasts were mixed and cultured without fusion. *2: Each species of protoplasts were fused and cultured separately. *3: Each species of protoplasts were fused separately and then mixed. *4: Two species of protoplasts were mixed and then fused (Somatic hybrid). Fused protoplasts were incubated in the medium of 1/2 MS medium (pH 5.7),1/4 NH4+,1% glucose, 0.4M mannitol, 0.1mg/l BA and 0.5 mg/l 2, 4-D.

Formation of Somatic Hybrid Callus between Chrysanthemum coccineum and Chrysanthemum coronarium L. war. Spatiosum Bailey (CHUUBA).