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Two cytoplasmic male sterile (CMS) lines were found in the progenies of an intergeneric cybrid (Brassica oleracea L. var. capitata) derived from asymmetrical protoplast-fusion and an interspecific chimera (Brassica rapa L. var. peruviridis) derived from in vitro grafting. Although these CMS lines were different in origin, they both had abnormal anthers and aborted pollen grains. The mitochondrial DNA in these CMS lines indicated the presence of orf138, an indicator gene for Ogura-type cytoplasm. In this study, direct sequencing of two regions near the rps16 in chloroplast DNA was performed. Although the sequence of the two regions in these two CMS lines was different from that in their parental lines, it was the same as the sequence in an Ogura-type CMS radish. Thus, these results suggest that the two CMS lines possess chloroplast DNA of the Ogura-type or a similar type as well as Ogura-type mitochondrial DNA. Furthermore, the region between rps16 and trnK was compared among species related to Brassica by PCR. The result indicated the presence of many sequence variations in this region and suggested that chloroplast DNAs could be distinguished by analyzing this region by PCR.

plant biotechnology

Comparative studies on chloroplast DNA in Ogura-type CMS lines derived from a cybrid (Brassica oleracea L. var. capitata) and a chimera (Brassica rapa L. var. peruviridis) and on a distinction of chloroplast DNA by PCR in Brassicaceae