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In conjunction with Ca 2 signaling, the subcellular localization of Ca 2 channels in various plant cells has been under intense scrutiny. The present study focused on the subcellular localization of OsTPC1, a putative voltage-dependent calcium channel in rice. A newly developed antibody to the linker domain of OsTPC1 was shown to bind this protein in vitro. Immunoblotting of a plasma membrane (PM)-enriched fraction obtained by aqueous two-phase partitioning, as well as indirect immunofluorescence confocal microscopy of rice protoplasts, supported the idea that OsTPC1 is predominantly localizes in the PM, at least of suspension-cultured rice cells.

Evidence for the plasma membrane localization of a putative voltage-dependent Ca2+ channel, OsTPC1, in rice